Effects of Platelet-derived Growth Factor on Expression of Matrix Metalloproteinases-2 in Vascular Smooth Muscle Cell of Rat

Aim To investigate the effects of platelet-derived growth factor(PDGF) on migration and matrix metalloproteinases-2(MMP-2) expression in vascular smooth muscle cells(VSMC).Methods After treated with PDGF 0 min,20 min and 6 hours,the ability of cell migration was measured using microscope,and MMP-2 activity was assayed by gel zymography.Furthermore the expression of MMP-2 was screened by using Chip and confirmed by reverse transcription polymerase chain reaction(RT-PCR).Results The ability of cell migration was improved by PDGF.It was 2.13-fold for 20 min treatment and 3.09-fold for 6 h treatment compared to without PDGF treatment,respectively(P0.05,n=3).The activity of MMP-2 was also significantly increased 1.62-fold and 2.87-fold for 20 min and 6 h treated by PDGF compared with 0 min,respectively(P0.01,n=3).The mRNA expression of MMP-2 was 1.54-fold,2.2-fold higher in cultured with PDGF at 20 min and 6 h than 0 min,respectively(P0.05,n=3).Conclusion Our data indicate that PDGF improve vascular smooth muscle cell migration via inducing MMP-2 expression and enhancing MMP-2 activity.