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2009•Chinese Journal of Laboratory DiagnosisRequires access

Analysis of Western Blot of heat shock protein 47 in rat acute spinal cord injury

Yang You-geng

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Abstract

Objective To study the changes of heat shock protein 47(HSP47) in rat acute spinal cord injury(SCI).Methods A modle of clip-compression injury of spinal cord in rats was used.27 Wistar rats were divided to normal group,sham operation group and SCI group randomly.Basso,Beattie,Bresnahan(BBB) Locomotor Rating Scale were assessed at 3days and every weeks after SCI.They were sacrificed to study the expression level of HSP47 using Western Blot technique at 1,3 and 5 weeks.Results There was a lower expression level of HSP47 in normal group and sham operation group.But,the expression level of HSP47 after SCI was higher.The level of HSP47 probably ascended after 5 weeks.Conclusion There is a higher level of HSP47 after SCI in Wistar rats.HSP47 is probably involved in pathological change after SCI.

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What this paper is about

Objective To study the changes of heat shock protein 47(HSP47) in rat acute spinal cord injury(SCI).Methods A modle of clip-compression injury of spinal cord in rats was used.27 Wistar rats were divided to normal group,sham operation group and SCI group randomly.Basso,Beattie,Bresnahan(BBB) Locomotor Rating Scale were assessed at 3days and every weeks after SCI.They were sacrificed to study the expression level of HSP47 using Western Blot technique at 1,3 and 5 weeks.Results There was a lower expression level of HSP47 in normal group and sham operation group.But,the expression level of HSP47 after SCI was higher.The level of HSP47 probably ascended after 5 weeks.Conclusion There is a higher level of HSP47 after SCI in Wistar rats.HSP47 is probably involved in pathological change after SCI.

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Available abstract

Objective To study the changes of heat shock protein 47(HSP47) in rat acute spinal cord injury(SCI).Methods A modle of clip-compression injury of spinal cord in rats was used.27 Wistar rats were divided to normal group,sham operation group and SCI group randomly.Basso,Beattie,Bresnahan(BBB) Locomotor Rating Scale were assessed at 3days and every weeks after SCI.They were sacrificed to study the expression level of HSP47 using Western Blot technique at 1,3 and 5 weeks.Results There was a lower expression level of HSP47 in normal group and sham operation group.But,the expression level of HSP47 after SCI was higher.The level of HSP47 probably ascended after 5 weeks.Conclusion There is a higher level of HSP47 after SCI in Wistar rats.HSP47 is probably involved in pathological change after SCI.

Key concepts: Western blot, Spinal cord, Spinal cord injury, Medicine, Heat shock protein, Pathological, Shock (circulatory), Pathology

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