Polyploid Induction and Ploidy Analysis of Anthurium andraenum Linden
Tian Li-min
Abstract
Tian Li-min
Abstract
[Objective] The study was to provide the reference for inducting polyploid of Anthurium andraenum Linden.[Method] With the reproduction block mass of aerial roots and seedings of A.andraenum as materials and the colchicine as the induced medical solution,3 methods of soaking,medium culture and threading were taken to induce the polyploid of reproduction block mass and seedlings of A.andraenum,the effects of different induction methods,colchicine concn.and induction time on induction rate of A.andraenum were studied.[Result] The polyploid cell could be obtained by 3 induction methods.When colchicine was in 0.1%-0.3%,the induction rate of polyploid was increased with the increment of inductive dose and the delay of induction time.When the reproduction block mass was treated with soaking method,the induction effect of polyploid was the best,and when the reproduction block mass were treated by 0.3% colchicine for 7 h,the induction rate reached 63.3%.[Conclusion] The optimum condition of polyploid induction for A.andraenum was that the reproduction block mass of aerial root was soaked by 0.3% colchicine for 7 h.
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[Objective] The study was to provide the reference for inducting polyploid of Anthurium andraenum Linden.[Method] With the reproduction block mass of aerial roots and seedings of A.andraenum as materials and the colchicine as the induced medical solution,3 methods of soaking,medium culture and threading were taken to induce the polyploid of reproduction block mass and seedlings of A.andraenum,the effects of different induction methods,colchicine concn.and induction time on induction rate of A.andraenum were studied.[Result] The polyploid cell could be obtained by 3 induction methods.When colchicine was in 0.1%-0.3%,the induction rate of polyploid was increased with the increment of inductive dose and the delay of induction time.When the reproduction block mass was treated with soaking method,the induction effect of polyploid was the best,and when the reproduction block mass were treated by 0.3% colchicine for 7 h,the induction rate reached 63.3%.[Conclusion] The optimum condition of polyploid induction for A.andraenum was that the reproduction block mass of aerial root was soaked by 0.3% colchicine for 7 h.
Key concepts: Polyploid, Colchicine, Reproduction, Ploidy, Biology, Botany, Genetics, Gene