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A new assessment method for animal model of choroidal neovascularization (CNV)

Qin Hua Xu

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Abstract

Objective: To establish a new assessment method for animal model of choroidal neovascularization(CNV).Methods: In this model,CNV was induced by laser photocoagulation in C57BL/6 mice with the krypton red laser.Animals from each group were sacrificed and were perfused through the heart with fluorescein-labeled dextran(FITC-Dextran) on day 1,3,5,and 7 post laser.Retinal pigment epithelium-choroid-scleral tissue,after using a mAb specific for elastin staining,was examined to determine the incidence and size of CNV complex by confocal microscopy.Results: Confocal micrograph of the neovascular complex in C57BL/6 mice showed the new vessels(green),and damaged Bruch's membrane(BM) and RPE were stained red for elastin.CNV appeared on day 3 post laser,reaching the peak on day 7 post laser.Conclusion: The new assessment method of animal model of choroidal neovasculation(CNV) is feasible,reliable and visualized.

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What this paper is about

Objective: To establish a new assessment method for animal model of choroidal neovascularization(CNV).Methods: In this model,CNV was induced by laser photocoagulation in C57BL/6 mice with the krypton red laser.Animals from each group were sacrificed and were perfused through the heart with fluorescein-labeled dextran(FITC-Dextran) on day 1,3,5,and 7 post laser.Retinal pigment epithelium-choroid-scleral tissue,after using a mAb specific for elastin staining,was examined to determine the incidence and size of CNV complex by confocal microscopy.Results: Confocal micrograph of the neovascular complex in C57BL/6 mice showed the new vessels(green),and damaged Bruch's membrane(BM) and RPE were stained red for elastin.CNV appeared on day 3 post laser,reaching the peak on day 7 post laser.Conclusion: The new assessment method of animal model of choroidal neovasculation(CNV) is feasible,reliable and visualized.

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Available abstract

Objective: To establish a new assessment method for animal model of choroidal neovascularization(CNV).Methods: In this model,CNV was induced by laser photocoagulation in C57BL/6 mice with the krypton red laser.Animals from each group were sacrificed and were perfused through the heart with fluorescein-labeled dextran(FITC-Dextran) on day 1,3,5,and 7 post laser.Retinal pigment epithelium-choroid-scleral tissue,after using a mAb specific for elastin staining,was examined to determine the incidence and size of CNV complex by confocal microscopy.Results: Confocal micrograph of the neovascular complex in C57BL/6 mice showed the new vessels(green),and damaged Bruch's membrane(BM) and RPE were stained red for elastin.CNV appeared on day 3 post laser,reaching the peak on day 7 post laser.Conclusion: The new assessment method of animal model of choroidal neovasculation(CNV) is feasible,reliable and visualized.

Key concepts: Choroidal neovascularization, Choroid, Elastin, Retinal pigment epithelium, Pathology, Animal model, Fluorescein, Medicine

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