Quantitative Analysis of Rat Choroidal Neovascularization Induced by Krypton Laser Photocoagulation Using Stretched Preparation of Choroidal Neovascularization

Objective To explore applied value of stretched preparation of choroidal neovascularization for quantitative analysis of rat choroidal neovascularization (CNV) induced by krypton laser photocoagulation.Methods Twenty adult Brown Norwey rats were divided into 4 groups.There were 5 rats in each group. Choroidal neovascularization was infused with high molecular weight FITC-dextran at d 7,14,21 and 28. Cornea, lens and nerve retina were removed and RPE-choroid-sclera flat mount was obtained. CNV was observed by fluorescence microscope. Area of CNV was measured by computer image analysis software.Results Peripapillary CNV induced by krypton laser photocoagulation was presented hyperfluorescence.Area of CNV gradually increased from d 7 to d 28(P0.01). Area of CNV at d 7,14,21 and d 28 was (12408±2988)μm~2,(28543±4422)μm~2,(32823±4028)μm~2,(34003±5126)μm~2 respectively that at d 14 was about 2 times of. area of CNV at d 7 (P0.01). No significant difference of area of CNV was seer at d 14,21 and d 28.Conclusions The stretched preparation of choroidal neovascularization is a reliable method and could use in the study of etiology, pathogenesis and therapeutic efficacy.