Cloning and Construction of Prokaryotic Expression Vector of VP1 Gene of Foot-and-mouth disease virus Serotype O

The RNA of FMDV serotype O was abstracted by Trizol.According to the published nucleotide sequence of the gene of foot-and-mouth disease virus serotype O,a pair of primers were designed and synthesized to clone the VP1 gene.The gene of FMDV was amplified by RT-PCR and subsequently inserted into the expression vector pET-32a.The VP1 gene was sequenced and compared with other published FMDV type O strains in the GeneBank.The VP1 gene was successfully conformed into the expression vector.