Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-mouth disease virus Serotype O in E.coli

According to the published sequence of VP1 gene of foot-and-mouth disease virus serotype O,a pair of primers were designed and synthesized.The VP1 gene was amplified by RT-PCR method from FMDV serotype O,and cloned into pET-28a vector.The prokaryotic expression plasmid containing VP1 gene was successfully constructed.The VP1 gene was sequenced and compared with the published sequence of VP1 gene of FMDV serotype O in the GenBank.The expression of recombinant plasmid pET-28a-VP1 in E.coli BL21 was induced and detected by SDS-PAGE and Western-blot analysis.The results showed that the structural protein VP1 gene of FMDV can express successfully in E.coli BL21.The fusion protein which was expressed in E.coli BL21 can be recognized by the positive serum of cattle that was infected by FMDV.