Levels Changes of 5 Subgingival Putative Periodontal Pathogens and the Promotion of Clinical Parameters following Periodontal Initial Therapy

Objectives: To investigate the level changes of porphyromonas gingivalis,tannerella forsythia,prevotella intermedia,Aggregatibacter actinomycetemcomitans and treponema denticola and the promotion of clinical parameters following periodontal initial therapy in the patients with chronic periodontitis.Methods: Twenty patients(forty sites)with chronic peridontitis volunteered in this study.Clinical parameters including PPD,CAL and BOP of the study sites were monitored and subgingival plaque samples were taken prior to therapy and after 3 months.All subjects received oral hygiene and full mouth SRP.Semi-quantification of P.gingivalis,T.forsythia,P.intermedia,A.actinomycetemcomitans and T.denticola were determined by polymerase chain reaction(PCR) and reverse hybridization assay.Results: After therapy,the promotions of PPD and BOP were significant(P0.001),and the promotion of CAL was not significant.The levels of P.gingivalis,T.forsythia and T.denticola were significantly reduced after therapy(P0.05 or P0.001).The level changes of 5 subgingival putative periodontal pathogens were different between the PPD≤6mm group and the PPD6mm group at the baseline.Conclusion: Periodontal initial therapy was an useful therapy for the chronic periodontitis patients to promote the clinical parameters and decrease the levels of P.gingivalis,T.forsythia and T.denticola effectively.But at the PPD6mm sites at the baseline,the influence of the periodontal initial therapy on the level changes of 5 subgingival putative periodontal pathogens was limited.