Effect of recombinant human IL-10 on skin grafting rejection and peripheral blood T lymphocyte subsets in rabbits

Objective To investigate the effect of recombinant human IL-10(rhIL-10) on skin grafting rejection and peripheral blood T lymphocyte subsets in rabbits. Methods Thirty-six white rabbits were equally divided into donor and-1-1-1-1recipient groups, and the recipient group was further allocated to rhIL-10 group(5 ig·kg ·d and 10 ig·kg ·d), cyclosporin-1-1-1-1-1-1-1-1(CsA) group(5 mg·kg ·d and 10 mg·kg ·d), rhIL-10(5 ig·kg ·d) + CsA(5 mg·kg ·d) group, and normal saline group(1 mL/d). The agents were continuously injected muscularly from 1 d pre-transplant to 8 d post-transplant. Skin graft + +rejection, and CD4 and CD8 cells and CD4/CD8 ratio from peripheral blood were determined. Results Onset of skin rejection was later in rhIL-10 group than that in normal saline group(P0.05), and so did in rhIL-10 + CsA group compared with low-dose +rhIL-10 and CsA groups(P0.05). CD4 cells and CD4/CD8 ratio increased during skin rejection, and peaked during skin graft necrosis. Conclusion rhIL-10 can inhibit the skin rejection in white rabbits, and CD4/CD8 ratio is useful for monitoring the skin rejection.