Cloning and Expressing of Avian Influenza Virus NS1 Gene in Procaryotic Cell

The complementary DNA of NS1 gene was prepared from the cDNA by RT-PCR.The amplified fragment was cloned into the plasmid pMD18-T.The sequence coding of signal peptide M was deleted.The PCR products and the expressing vector were digested with restriction endonuclease EcoRⅠ and Hind Ⅲ,then the NS1 gene was inserted into the pET-32a system,after that,the constructed recombinant plasmid was transformed into BL21(DE3).The recombinant protein was expressed and identified by SDS-PAGE and Western blot,which proved it has good biological activity.