Cloning Expression and Antibody Preparation of Fused Gene NS1A of H9N2 Subtype Avian Influenza Virus

In this research,we got fused gene NS1A through overlapped PCR,and inserted fused gene into prokaryotic expression vector pET-20b successfully,then transformed recombinant plasmid into competent BL21,fusion protein NS1A expressed after IPTG induction.Expression protein was detected by denaturing SDS-PAGE and western blotting.The result showed that protein expressed at high efficiency.Thin-layer scanning analysis indicated interest protein accounted for 15 percent in gross proteins.We immunized rabbits with fusion protein NS1A.Using indirect-ELISA,antibody titer was detected to be 1∶25 600.This research layed foundation on detecting eukaryotic expression product of fused gene NS1A.