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2002•Journal of Nantong MedicalRequires access

THE ISOLATION,CULTURE OF THE MSCs OF BONE MARROW AND STUDY ON THEIR BIOLOGICAL FEATURES IN VITRO

Jian Zhao

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Abstract

ObjectiveTo investigate the behavior of MSCs in vitro.Methods:MSCs were extracted from bone marrow and purified.The isolated cells were observed with phase-contrast microscope and electronic microscope.Mesenchymal stem cells were extracted from the bone marrow in blood and were proliferated in vitro.Then they were observed under the phase-contrast microscope and electronic microscope and the growth curve was drawn accordingly.Results:(1)MSCs that are in low abundance can grow quickly when cultured in vitro.(2)MSCs have the tendency to be the fibroblasts.(3)When cultured in mono-layer,those attached MSCs showed actavism after some subculture.(4)The structures of the organellae varied with the changes of cell biological characteristics.Conclusion:The success of in vitro culture of MSCs will be useful for amendment of skeletal tissue defects by tissue engineering aftermath.

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What this paper is about

ObjectiveTo investigate the behavior of MSCs in vitro.Methods:MSCs were extracted from bone marrow and purified.The isolated cells were observed with phase-contrast microscope and electronic microscope.Mesenchymal stem cells were extracted from the bone marrow in blood and were proliferated in vitro.Then they were observed under the phase-contrast microscope and electronic microscope and the growth curve was drawn accordingly.Results:(1)MSCs that are in low abundance can grow quickly when cultured in vitro.(2)MSCs have the tendency to be the fibroblasts.(3)When cultured in mono-layer,those attached MSCs showed actavism after some subculture.(4)The structures of the organellae varied with the changes of cell biological characteristics.Conclusion:The success of in vitro culture of MSCs will be useful for amendment of skeletal tissue defects by tissue engineering aftermath.

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Available abstract

ObjectiveTo investigate the behavior of MSCs in vitro.Methods:MSCs were extracted from bone marrow and purified.The isolated cells were observed with phase-contrast microscope and electronic microscope.Mesenchymal stem cells were extracted from the bone marrow in blood and were proliferated in vitro.Then they were observed under the phase-contrast microscope and electronic microscope and the growth curve was drawn accordingly.Results:(1)MSCs that are in low abundance can grow quickly when cultured in vitro.(2)MSCs have the tendency to be the fibroblasts.(3)When cultured in mono-layer,those attached MSCs showed actavism after some subculture.(4)The structures of the organellae varied with the changes of cell biological characteristics.Conclusion:The success of in vitro culture of MSCs will be useful for amendment of skeletal tissue defects by tissue engineering aftermath.

Key concepts: Mesenchymal stem cell, In vitro, Subculture (biology), Bone marrow, Phase contrast microscopy, Pathology, Biology, Cell biology

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THE ISOLATION,CULTURE OF THE MSCs OF BONE MARROW AND STUDY ON THEIR BIOLOGICAL FEATURES IN VITRO — Research Paper | ScholarLens