BIOLOGICAL CHARACTERISTICS OF RABBIT BONE MARROW MESENCHYMAL STEM CELLS CULTURED IN VITRO

Objective: Isolated cultures of rabbit bone marrow mesenchymal stem cells (MSCs) were made to study their biological characteristics in vitro. Methods: MSCs were isolated from bone marrow by density gradient centrifugation. Then the MSCs were purified and cultured. The appearance of MSCs was observed under the phase contrast microscope. Microstructure of the MSCs was observed under transmission electron microscope. The cell growth curve was drawn and MTT assay was tested to study the reproductivity and metabolic activity of MSCs. Results: Under the phase contrast microscope, the appearance of the MSCs was spindle like, and there were a large number of mitochondrion and rough endoplasmic reticulum in the nucleus of MSCs. Conclusion: It's easy to obtain materials for the experiment in obtaining MSCs in a large number.;