Effects of shenfu injection on expression of NF-κB,TNF-α and ICAM-1 after renal ischemia reperfusion in rats

AIM: To explore the effect of shenfu injection on renal ischemia reperfusion injury in rats and to observe the expression of nuclear factor kappa B(NF-κB),tumour necrosis factor-α(TNF-α) and intercellular adhension molecule-1(ICAM-1).METHODS: 36 SD rats were divided into three groups randomly.There were 12 rats in each group.Renal ischemia reperfusion injury was induced by left renal pedicle occlusion for 45 minutes,followed by reperfusion with contralateral nephrectomy in rats.In shenfu pretreatment group,the rats were pretreated 1 hour before ischemia with shenfu injection.In ischemia/reperfusion group and control group,the animals were both pretreated with 0.9% natrill chloride,but in control group,the left renal pedicle was isolated,however,occlusion of the pedicle was not performed.The blood and kidney tissue were harvested after 2 hours of reperfusion.The serum creatinine(Cr) and blood urea nitrogen(BUN) values were measured with an automatic analyzer.Immunohistochemistry were used to evaluated the expression levels of NF-κB and ICAM-1,the concentration of tumor necrosis factor-α(TNF-α)in the kidney and plasma was determined by enzyme-linked immunoadsordent assay(ELISA).RESULTS: Compared with control group,BUN and Cr were gradually increased in ischemia/reperfusion group(P0.01).The average OD values of NF-κB and ICAM-1 protein were significantly higher in ischemia/reperfusion group than those in shenfu pretreatment group(P0.01) and control group(P0.01).The levels of TNF-α in the kidney tissue and plasma were markedly increased after reperfusion.By contrast,the levels of TNF-α in shenfu pretreatment group were significiantly lower than those in ischemia/reperfusion group(P0.05).CONCLUSION: The results show that SF has a protective effect against renal ischemia reperfusion injury,which may through inhibiting the activity of NF-κB and the expression of ICAM-1 and TNF-α.