Influence of ShenFu Injection on Expressions of NF-κB p65 and COX-2 in Aged Myocardial Ischemia Reperfusion Rat Model

Objective: To study the effects of ShenFu injection on expressions of NF-κB p65 and COX-2 in aged rat model with myocardial ischemia reperfusion.Method: Thirty aged wistar rats were randomly divided into three groups(n=10 each): sham group(S group);ischemia reperfusion group(I/R group) and ShenFu group(SF group).Model with myocardial ischemia reperfusion was established through left anterior descending coronary artery ligation.Influence of ShenFu injection on spillage of creatine phosphokinase isoenzyme(CK-KB),aspartate amino transferase(AST) and lactate dehydrogenase(LDH) in serum of aged rat with ischemia reperfusion were observed;activity of NF-κB p65 and expression of COX-2 in myocardial tissue after reperfusion were observed with immunohistochemical method.Result: Spillage of CK-KB,AST and LDH in serum of aged rats in I/R group decreased significantly.Compared with S group,activity of NF-κB p65 and protein expression of COX-2 improved in myocardial tissue of I/R group(P0.05).Above indexes in SF group dropped down remarkably.Conclusion: High level expression of NF-κB p65 and COX-2 demonstrated that inflammation participate in myocardial ischemia reperfusion.The mechanism might be NF-κB p65 play an important role through transferring and regulating COX-2.ShenFu injection can inhibit the activity of NF-κB p65,reduce expression level of COX-2,alleviate inflammatory reaction and protect myocardial muscle.