The Research on Expression of ApoJ mRNA in Rat Cerebral Infarction Models

Objective To investigate the mechanism of the neuroprotective effect of ApoJ in rat acute cerbral infarction.Methods 70 healthy male SD rats were divided randomly into normal control group,shamoperated group,cerebral infarction 6 hour group,24 hour group,3 day group,5 day group and 7 day group.Permanent cerebral infarction models were prepared by occluding the middle cerebral artery(MCA) using thread embolizing,the C3 and ApoJ mRNA expression was analyzed by RT-PCR.Results An increased expression was found in ischemia rat brain and a low level expression ApoJ mRNA was detected in normal rat brain,a positive correlation found between C3 mRNA and ApoJ mRNA expression(r = 0.427,P0.01).Conclusions Increased ApoJ expression may be invoved in neuroprotection in cerebral infarction by inhibiting the complement activation.