Effect of lactuside B on expression of Grp78 and Caspase-3 mRNA in rats' cerebral cortex after cerebral ischemia reperfusion injury

AIM To investigate the influences of lactuside B on the mRNA expression of Grp78 and Caspase-3 in rats' cerebral cortex after cerebral ischemia injury. METHODS First,middle cerebral artery ischemia-reperfusion injury model was established. Each group was then treated with its corresponding medicine. Animals were separately sacrificed at 24 h and 72 h and the mRNA levels of Grp78 and Caspase-3 in rats' cerebral cortex were checked by RT-PCR. RESULTS Compared with the model group,all doses lactuside B treatment can increase the expression of Grp78 mRNA in rats' cerebral cortex. Moreover,the expression levels of Grp78 mRNA were higher in medium and low dose groups than those in the model group( P 0. 05). Generally,the effects of low and medium groups were better than those of the positive control medicine and Nimodipine group( P 0. 05). Compared with the model group,receving lactuside B treatment could degrade the expression level of Caspase-3 mRNA( P 0. 05). Furthermore,the effects of medium and high dose groups were better than that of Nimodipine group( P 0. 05). CONCLUSION Lactuside B can play a positive role in resisting cerebral ischemia by increasing the expression of Grp78 mRNA and decreasing the expression of Caspase-3 mRNA.