Effect of MicroRNA-155 on laryngeal squamous cell carcinoma Hep-2 cells

OBJECTIVE To investigate the effect of microRNA-155(miR-155) over-expression on malignant phenotype in Hep-2 cell line of laryngeal squamous cell carcinoma.METHODS miR-155 mimics were transfected into Hep-2 cells by Lipofectamine RNAiMAX.miR-155 expression level was detected by qRT-PCR.Cell proliferation were evaluated by CCK8 assay and colony formation.Cell apoptosis were analyzed by flow cytometry and Hoechst 33342 propidium iodide staining.Cell migration was detected by wound healing assay.RESULTS After transfection of miR-155 mimics into Hep-2 cells,the data of qRTPCR showed that miR-155 expression level was significantly up-regulated.CCK-8 assay and colony formation revealed that growth or proliferation ability of miR-155 group was obviously promoted.Cell apoptosis or death was suppressed after over-expressed miR155 which was analyzed by flow cytometry and Hoechst 33342 propidium iodide staining.Remarkable enhancement of cell migration was observed in miR155 group which was detected by wound healing assay.CONCLUSION Over-expression of miR-155 could induce proliferation and migration of Hep-2 cell line,and inhibit apoptosis.It might play the function as an oncogene in the development of laryngeal squamous cell carcinoma.