Effects of Seed Soaking in Choline Chloride Solution on Seeds Germination and Some Physiological Characteristics of Spinach Seedlings

[Objective] The spinach seeds were soaked with choline chloride(CC) solution of different concentration,and the effects of CC on seeds germination and some physiological characteristics of spinach seedlings were studied.[Method] The germination rate and germination index,soluble protein,solube sugar,chlorophyll content,antioxidant enzyme activity,production rate of O2· and MDA content were measured.[Result] CC could increase germination rate and germination index of spinach seeds.It also could increase soluble sugar,soluble protein and chlorophyll content of spinach seedlings.The superoxide dismutase,the calatase and the peroxidase activities also was increased after soaking with CC solution.Moreover,the production rate of O2· and MDA content decreased markedly contrasting with spinach seedlings without CC treatment.[Conclusion] The effects on seeds germination and some physiological characteristics of spinach seedlings were best after treatment by CC with the concentration of 25 mg/L.