Study on Anti-tumor Effects of CIK Cells in Tumor Immunol Therapy

Objective Study the proliferation and anti-tumor effects of cytokine induced killer cells (CIK cells) in tumor immunol therapy. Methods CIK cells were generated by culturing PBMNC in the presence of IFN-r, IL-2 and anti-CD3 McAb. CIK ultures were analyzed on different time points by FACS. The killing efficacy in tumor cell line(K562) were detected by MTT assay. To treat 30 patients suffered from malignant tumors with CIK cells. The function of cell immunity in patients peripheral blood were measured by immunofluorescence before and after 2 weeks of treatment respectively. Results After 2 weeks of culture, CIK cells expanded about 30 folds. The percentage of CD3+ ,CD3+CD4+,CD3+ CD8+,CD3+CD56+ cells increased significantly after cytokines induction (P0.01).CIK cells had great cytotoxity against K562 cell line in vitro ,the median inhibitory rate was 50%. The percentages of CD3+, CD3+CD4+,CD3+CD8+,CD3+CD56+ of post-treatment patients changed significantly compared with that in the no-treatment group (P0.05). Conclusion CIK cells have a stronger significsnt suppression effect on growth of K562 cell line in vitro. It also could improve immune function of patients .The results all provide an basis for CIK cells in clinical application as a adaptive immunotherapy.