The Therapy of Cytokine-Induced Killer Cells and its Effects on Immune Functions of the Patients with Malignant Tumor

OBJECTIVE To study the proliferation profile of CIK cells from patient's peripheral blood with different advanced malignant tumors; and assess its effects on immune functions of those patients. METHODSCIK cells were induced from PBMC of 12 tumor patients by in vitro incubation with IL-2?γ-IFN?anti-CD3 monoclonal antibody and IL-1, then were transfused back to those patients;the T cell subgroup and NK cell of patients before and after CIK cell treatment were detected with FCM. RESULT: After 13 days culture, the total number of CIK cells and CD3~(+)CD56~(+)T cells were expanded 123 (range 46～301) and 1 656 (177～5130) times respectively. After being treated with auto-CIK cells, the CD3?CD4?T and NK cells of those patients were increased significantly(P0.01). CONCLISIONCIK cells may be expanded effectively from patients with different malignant tumor;adoptive immunotherapy of auto-CIK cells can significantly enhance cellular immune functions and the side effect is rare, so it is a promising therapy.