Cloning and Expression Analysis of CBF1 from Cucumber (Cucumis sativus L.)
Xin Jiang
Abstract
Xin Jiang
Abstract
The CBF1 gene were obtained by RT-PCR from cucumber(Cucumis sativus).The sequence showed high homology(99.44%) to the published sequences of CBF1.The expression patterns of the CBF1 gene in response to low temperature,drought,ABA and NaCl stresses at different time points were further investigated by using RT-PCR.The results showed that CBF1 gene could express under low temperature and NaCl treatment in cucumber.However,it could not express under drought and ABA treatment.
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The CBF1 gene were obtained by RT-PCR from cucumber(Cucumis sativus).The sequence showed high homology(99.44%) to the published sequences of CBF1.The expression patterns of the CBF1 gene in response to low temperature,drought,ABA and NaCl stresses at different time points were further investigated by using RT-PCR.The results showed that CBF1 gene could express under low temperature and NaCl treatment in cucumber.However,it could not express under drought and ABA treatment.
Key concepts: Cucumis, Cloning (programming), Gene, Biology, Gene expression, Homology (biology), Botany, Sequence homology