Genetic Diversity and Relationship of 30 Tea Plant Germplasms in Sichuan Revealed by SRAP Marker

Sequence related amplified polymorphism(SRAP) marker was applied to analyze genetic diversity and relationship of 30 tea plant germplasms in Sichuan to provide references for evaluation and identification of tea plant germplasms,screening of core germplasms,genetic improvement,resource protection and utilization.The results showed that eleven pairs of SRAP primers produced 2 744 amplified fragments.Of which 2474 were polymorphic.The polymorphism information index ranged from 0.22 to 0.36 with an average of 0.30.The Nei’s gene diversity index was 0.342 1～0.545 5 with an average of 0.4227.The average Shannon’s information index was 0.271 1,ranging from 0.204 3 to 0.371 0.These findings revealed that the germplasms had a great deal of genetic diversity.The dendrogram showed distinct genetic differentiation among germplasms.The genetic similarity coefficient varied from 0.583 to 0.919.The UPGMA cluster analysis showed that 30 tea plant germplasms could be clustered into A and B groups when the genetic similarity coefficient was given as 0.66.