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2004•Xi'nan nongye xuebaoRequires access

Genetic analysis and indentification of maize by RAPD markers

Tao Gang, Liu Zou-yi, Ying Zhu, Song Ji-xuan, Zehui Chen

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Abstract

Nineteen maize varieties were analyzed by RAPD with 26 ten-mer random primers, of which, 10 random primers were selected for RAPD analysis because of their good polymorphism. The results showed that primers S7, S461, S495 and S506 could be successfully used to identify nineteen maize varieties by the RAPD profile. The phylogenetic tree of nineteen maize varieties constructed by cluster analysis indicated that the pedigree between the parents and their hybrid progeny was right.

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What this paper is about

Nineteen maize varieties were analyzed by RAPD with 26 ten-mer random primers, of which, 10 random primers were selected for RAPD analysis because of their good polymorphism. The results showed that primers S7, S461, S495 and S506 could be successfully used to identify nineteen maize varieties by the RAPD profile. The phylogenetic tree of nineteen maize varieties constructed by cluster analysis indicated that the pedigree between the parents and their hybrid progeny was right.

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Available abstract

Nineteen maize varieties were analyzed by RAPD with 26 ten-mer random primers, of which, 10 random primers were selected for RAPD analysis because of their good polymorphism. The results showed that primers S7, S461, S495 and S506 could be successfully used to identify nineteen maize varieties by the RAPD profile. The phylogenetic tree of nineteen maize varieties constructed by cluster analysis indicated that the pedigree between the parents and their hybrid progeny was right.

Key concepts: RAPD, Biology, Phylogenetic tree, Phylogenetic relationship, Genetics, Veterinary medicine, Genetic diversity, Gene

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