Study on the Lethal Effect and Mechanisms of Oxaliplatin and UCN-01 on HCT116 Colon Cancer Cell Line

Objective: To observe the growth inhibitory effect of Oxaliplatin(L-OHP) and(7hydroxystaurosporine,(UCN-01) on HCT116 colon cancer cell line in single or combination treatment,and to investigate its mechanisms.Method: The growth inhibitory effect was evaluated by MTT assay in single agent or combination treatment.Apoptosis was investigated by flow cytometry and Western blotting,and mitotic catastrophy was determined by HE staining.Result: Combination treatment of L-OHP in low concentration and UCN-01 has synergistic effect on killing HCT116 colon cancer cell line,which was more effective than single treatment.AnnerxinV positive cell rate showed no difference between combination treatment or UCN-01 alone.Cellular morphology of HCT116 cell was changed in combination treatment,and megakaryocytes and polykaryocytes in combination treatment were increased by approximately 10% compared with the single treatment.Conclusion: Combinition treatment of L-OHP and UCN-01 has synergistic inhibitory effect on HCT116 cell proliferation through inducing cell apoptosis and mitotic catastrophy.