Effects of resveratrol on the sequential expression of SIRT1 in cardiomyocytes

Objective To observe the effect of resveratrol(RES),an activator of silent information regulator 2,on the sequential expression of SIRT1 in cardiomyocytes.Methods After 48 h culture under normal condition,the embryonal rat heart-derived H9c2 cells were treated with 0(control group),10(low dose group),20(medium dose group)50 and 100 μmol/L(high dose groups)of RES for 0,12,24,36 and 48 h.Cell viability was analyzed by MTT assay,expression of SIRT1 mRNA by RT-RCR and SIRT1 products by Western blot.Results The optical densities(A)of MTT was increased in the low and medium dose groups,appeared markedly higher with 20 μmol/L RES treat-ment(P0.05),and was lower than normal in the high dose groups,particularly in the 100 μmol/L group(P0.05).The expressions of SIRT1 mRNA and protein were increased after treated with different concentrations of RES(P0.05),most notability with 20 μmol/L.At different time spots of incubation with 20 μmol/L RES(0,12,24,36,and 48 h),the A values of SIRT1 mRNA and proteins were higher than those in controls(P0.05),with a peak at 24 h.Conclu-sion The maximum expression of SIRT1 was achieved with treatment of 20 μmol/L RES for 24 h in cadiacmyocytes.