Optimal Extraction Processing of Corn Germ Oil through Water-Enzymolysis Method

Water-enzymolysis method was used to extract corn germ oil. The optimal extraction processing parameters were explored by single-factor and orthogonal experiments to be material-liquid ratio of 1:5 (g/mL), compound enzyme addition amount of 2.5%, enzymatic hydrolysis time of 7 h and hydrolysis pH of 6.0. The compound enzyme was composed of cellulase and α-amylase with a mass ratio of 4:3. Under the optimal extraction processing conditions, the extraction rate of corn germ oil was up to 89%. Gas chromatography analysis indicated that the contents of oleic acid and linoleic acid in corn germ oil were 43.46% and 40.22%, respectively.