Long-term observation of CCL_4-induced live cirrhosis in rat

Objectives To establish a rat persistent liver cirrhosis model.Methods Fore stablishing a liver cirrhosis induced by CCl_4 plus resection of liver median lobe in rat,200 three-month-old male Wistar rats were randomly divided into normal(A),CCl_4(B)and CCl_4/resection of liver median lobe(C)group.The rats were batched and sacrificed every three months.The spleen weights,portal vein widths,testes weights,the status of hepatic fibrosis and liver cirrhosis were assessed.Results The spleen weights and portal vein widths were increased from A to C group,whereas there was no difference in testes weights among those three groups.The rate of maintained status of hepatic fibrosis and liver cirrhosis was significantly higher in the C group than in the B group(P0.01).The former was 92% and 100% and the latter 50% and 75%.Conclusions It is steady to establish a rat liver cirrhosis model induced by CCl_4/resection of liver median lobe for a relatively long times.