Expression of VP1 Gene of FMDV Type O in Recombinant Baculovirus

According to the VP1 gene sequence of FMDV type O,two primers of VP1 were designed, and the expected fragment of 633 bp was obtained and cloned into pMD-18T vector. Then the VP1 gene was cloned into baculovirus expression vectors pFastBacⅠ, and the recombinant plasmid of pFastBac-VP1 was constructed.The plasmid was transformed to DH10Bac competent cell, and screened by three antibiotics and blue-white patch. The white colony was the recombinant Bacmid-VP1. And then the Bacmid-VP1 was transfected into the Sf9 insect cells, by three passaging the recombinant baculovirus was obtained. Finally, the expressed product were detedted by SDS-PAGE and Western-blotting. The results showed that the VP1 protein was high-efficiently expressed in the recombinant baculovirus.