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2004•The Chinese Journal of Modern Applied PharmacyRequires access

Study on HPLC fingerprint of radix glycyrrhizae

Jin YongXin, Yao Linqing, Shilan Feng, Xiaojun Li, Lian‐Ping Wang

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Abstract

OBJECTIVE To study HPLC-fingerprint of Radix Glycyrrhizae. METHOD HPLC with C 18 [4.6(μm)×250mm]column was used,the methanol-20% acetonitrile with 0.5% glacial acetic acid was used as mobile phase and the detection wavelength was 254nm.RESULTS Indicating 28 peaks in common,and the HPLC fingerprint of Radix Glycyrrhizae was eatablished.CONCLUSION This method is steady and accurate with a good repeatability and can be used as a quality control for Radix Glycyrrhizae.

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What this paper is about

OBJECTIVE To study HPLC-fingerprint of Radix Glycyrrhizae. METHOD HPLC with C 18 [4.6(μm)×250mm]column was used,the methanol-20% acetonitrile with 0.5% glacial acetic acid was used as mobile phase and the detection wavelength was 254nm.RESULTS Indicating 28 peaks in common,and the HPLC fingerprint of Radix Glycyrrhizae was eatablished.CONCLUSION This method is steady and accurate with a good repeatability and can be used as a quality control for Radix Glycyrrhizae.

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Available abstract

OBJECTIVE To study HPLC-fingerprint of Radix Glycyrrhizae. METHOD HPLC with C 18 [4.6(μm)×250mm]column was used,the methanol-20% acetonitrile with 0.5% glacial acetic acid was used as mobile phase and the detection wavelength was 254nm.RESULTS Indicating 28 peaks in common,and the HPLC fingerprint of Radix Glycyrrhizae was eatablished.CONCLUSION This method is steady and accurate with a good repeatability and can be used as a quality control for Radix Glycyrrhizae.

Key concepts: Radix (gastropod), Chromatography, High-performance liquid chromatography, Repeatability, Fingerprint (computing), Chemistry, Gradient elution, Acetic acid

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