The effects of gronulocyte colony-stimulating factor on autologous peripheral blood stem cell mobilization in elderly patients with acute myocardial infarction

Objective To observe the mobilization effects of autologous circulating blood stem cell by granulocyte colony-stimulating factor(G-CSF)in elderly patients with acute myocardial infarction(AMI).Methods Twenty patients with AMI who received inclusive type G-CSF with the dose of 300~600 μg/d,were allocated to two groups.One was elderly group(≥70 years old),the other was young group(70 years old).The patients received G-CSF by hypodermic injection for 5 days.In the process of the mobilization of the circulating blood stem cell,the white blood cell(WBC)counts were measured and CD34~(+)cell counts in the circulating blood were determined by flow cytometer,and the complications related to the mobilization also were observed. Results In elderly group,before and the 3rd,4th,5th,6th,7th day after applying G-CSF,WBC counts(×10~9/L)were 6.75,28.16,34.93,34.40,38.93,21.85;CD34~(+)cell counts(×10~(6))were 6.25,51.10,92.60,109.65,134.69, 45.09 respectively.In young group,before and the 3rd,4th,5th,6th,7th day after applying G-CSF,WBC counts(×10~(9)/L)were 8.73,28.21,33.67,33.95,32.93,18.71;CD34~(+)cell counts(×10~(6)/L)were 9.01,71.79, 128.54,268.17,249.51,72.25 respectively.The WBC and CD34~(+) cell counts changed with CSF-applying days and their curve peaks were at the 6th day in the elderly group,and at the 5th day in young group.CD34~(+)cell counts was positive correlated with white blood cell counts in the circulating blood(r=0.94).No severe complications happened.Conclusions In elderly patients with AMI,the stem cell mobilization by G-CSF is effective with.the peak of mobilized white blood cell and CD34~(+)cell counts at the 6th day.CD(34)~(+)cell count is positive correlated with white blood cell count in the circulating blood.