Clinical observation on scheme optimization of mobilization effects of granulocyte colony-stimulating factor on stem cells

Objective Observe the mobilization effects of granulocyte colony-stimulating factor (G-CSF) on stem cells in patients with acute myocardial infarction (AMI), so as to find out an optimized scheme. Methods Forteen patients with AMI were randomly allocated to receive G-CSF to mobilize the stem cell,with either 300 μg/d (group A) or 600 μg/d (group B). The patients received G-CSF by hypodermic injection,and the duration was 5 days. In the process of the mobilization of the circulating blood stem cell, the white blood cell (WBC) and CD34+ cell counts in the circulating blood had been observed. Results Prior to applying G-CSF and the 2nd, 3rd, 4th, 5th, 6th, 7th, and 8th days after applying G-CSF, the counts of WBC had no significant difference between this two groups; but the 3rd, 5th, 6th, and 7th days after applying G-CSF, the counts of CD34+ cell had significant difference between this two groups (P=0.007, 0.019, 0.011, 0.005), CD34+ cell count in the circulating blood of group B was higher than that of groupA; the WBC and CD34+ counts peaked on the 5th day after applying G-CSF; CD34+ cell count in the circulating blood was positively related with the WBC count in the circulating blood (r=-0.659), was negatively related with body weight of patients (r=-0.356), not related with sex, age, or the onset time of AMI. Conclusion In patients with AMI, mobilization effect of G-CSF 600 μg/d on stem cells is better than that of G-CSF 300 μg/d.