Optimization of extraction process for total flavonoids from Radix Codonopsis Pilosulae by response surface methodology

Objective:To study the extracting process for flavonoids from Radix Codonopsis Pilosulae.Methods:In basis of ethanol concentration,ratio of material to liquid,extraction time and extraction temperature four single factor test,the optimal conditions for extracting flavonoids from Radix Codonopsis Pilosulae were determined by using response surface method.Results:The optimal extraction condition was as follows:extraction time was 68 min,concentration of ethanol was 90%,solid to solvent ratio was 1∶40,extraction temperature was 85 ℃.Under these conditions,the measured extraction rate of flavonoids was 0.839%,the relative error was 0.60% compared with the predictive value of 0.834%.Conclusion:It is feasible to adopt response surface methodology to optimize the extraction process for flavonoids from Radix Codonopsis Pilosulae.