Surveillance of antimicrobial resistance in clinical isolates from Tongji Hospital in Wuhan in 2005

Objective To investigate the resistance of clinical isolates to antimicrobial agents commonly used in Tongji Hospital during 2005.Methods Disk diffusion susceptibility test was used to determine the antimicrobial susceptibility.Data were analyzed by WHONET 5.3 software.Results A total of 3 119 clinical isolates were colleted.The top six pathogens were E.coli,P.aeruginosa,coagulase-negative Staphylococcus,Klebsiella spp,Acinetobacter spp.and S.aureus.S.maltophilia ranked tenth.About 53.7% of S.aureus isolates were identified as MRSA,74.8% of coagulase-negative Staphylococcus isolates were MRCNS.No strain of gram-positive cocci was found resistant to glycopeptides.The resistance rates of Enterobacteriaceae to carbapenem,piperacillin-tazobactam,cefoperazone-sulbactam,amikacin were less than 30%.And not more than 30% of non-fermentative bacilli were resistant to carbapenem or cefoperazone-sulbactam.ESBLs-producing strains accounted for 58.2% in E.coli and 44.4% in Klebsiella spp.The resistance rates of P.aeruginosa to ceftazidime,cefepime,ciprofloxacin ranged from 26.6% to 28.6%.The resistance rates of S.maltophilia to the third generation cephalosporins,flaoroquinolones ticarcillin-clavulanic,and minocycline ranged from 6.2% to 22.7%.Conclusions Two thirds of these clinical isolates are gram-negative bacilli,for which carbapenems are highly active.The resistance to most antimicrobial agents is increasing in the clinical isolates from Tongji Hospital.