Extraction and Determination of Total Flavonoids from Portulaca Oleracea L.in Yan an
Feng Xiao
Abstract
Feng Xiao
Abstract
Through ultrasionic cell-break method and Soxhlet extractions to extract the flavonoids in Portulaca oleracea L.and determinate its contents by UV spectrophotometry,the best condition of the extraction was selected by using orthogonal experiment.The results showed that factors influencing the extraction were in the order as follows:solid-liquid ratioethanol concentrationextractive time;the optimal extracting conditions extracted the flavonoids in Portulaca oleracea L.by the Ultrasionic cell-break method and Soxhlet extractions is:solid-liquid ratio 1:50(g/mL),ethanol concentration 90%and extractive time 40min.The contents of flavonoids from the Portulaca oleracea L.in Yan'an were 5.63%.
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Through ultrasionic cell-break method and Soxhlet extractions to extract the flavonoids in Portulaca oleracea L.and determinate its contents by UV spectrophotometry,the best condition of the extraction was selected by using orthogonal experiment.The results showed that factors influencing the extraction were in the order as follows:solid-liquid ratioethanol concentrationextractive time;the optimal extracting conditions extracted the flavonoids in Portulaca oleracea L.by the Ultrasionic cell-break method and Soxhlet extractions is:solid-liquid ratio 1:50(g/mL),ethanol concentration 90%and extractive time 40min.The contents of flavonoids from the Portulaca oleracea L.in Yan'an were 5.63%.
Key concepts: Portulaca, Extraction (chemistry), Chemistry, Chromatography, Botany, Biology