Approach of gastric carcinoma cells proliferation inhibited by celecoxib

Objective To observe the depression effects induced by celecoxib on gastric carcinoma BGC-823 cells proliferation induced by bFGF,to investigate the mechanism of inhibition induced by celecoxib on tumour cell proliferation.Methods After treatment of different dosages of celecoxib,expressions of COX-2,NF-κB,VEGF protein in gastric carcinoma BGC-823 cells induced by 25?ng/mlbFGF were measured using western blotting.Results 25?ng/ml bFGF promoted BGC-823 cell proliferation and expressions of COX-2,NF-κB,VEGF protein in a time-dependence way by PKB signal transduction(P0.01).Celecoxib with different concentrations had proliferation-inhibiting effect on BGC-823 cell and induced apoptosis.Different dosages of celecoxib had depressant effects on the expressions of COX-2,NF-κB,VEGF protein in BGC-823 cell induced by 25?ng/ml bFGF(P0.01).Conclusion celecoxib had determinate depressant effects on gastric carcinoma BGC-823 cells proliferation induced by bFGF.