Study on the effects of Cinobufacin combined with taxol on murine S180 tumor in vivo

OBJECTIVE To study on the effects of Cinobufacin combined with taxol injection on S180 tumor in vivo.METHODS Tumors were established by injection of S180 cells(1×106 cells per animal,to form S180 ascites or subcutaneously solid tumor in ICR male mice).Cinobufacin(80,20,5mg·kg-1) and it combined with taxol(80,20,5mg·kg-1+5mg·kg-1,respectively) were administrated ip for consecutive 9 days.In mice bearing S180 ascites,the prolongation rate of life were recorded.In mice bearing S180 sarcoma,mice were sacrificed at day 10 and tumor weight were recorded.RESULTS In mice bearing S180 ascites,the survival times of Cinobufacin groups were(21.5±2.4)days(P0.05),(18.6±1.4)days,(18.3±2.4) days,compared with the control(normal saline)group in which the survival times was(17.3±1.3)days.The survival times of combined groups were(26.6±1.6)days(P0.001),(24.3±2.1)days(P0.001),(21.8±1.1)days(P0.05).The prolongation rate of life were 56.4%,40.4% and 26.0% respectively.The tumor weight of Cinobufacin group were(1.05±0.48)g(P0.01),(1.44±0.41)g,(1.39±0.60)g,compared with the control(normal saline)group in which the tumor weight was(1.55±0.52)g.The tumor weight of combined groups were(0.61±0.40),(0.88±0.38),(1.09±0.55)g,and the tumor inhibition rate were 60.7%,43.3% and 30.0 respectively.Conclusion Cinobufacin combined with taxol could inhibit the proliferation of cancer cells efficiently.