Effect of different doses of sustained-release fluorouracil on mice bearing H22 ascitic tumor intraperitoneally

Objective To investigate the effect of sustained-release fluorouracil(Sinofuan) injected intraperitoneally on the mice with ascitic tumor.Methods Mouse models bearing ascites tumors were established via injecting H22 cells intraperitoneally.Sixty-eight model mice were assigned into 4 groups randomly,the control group,the low-dose(2mg),medium-dose(4mg),high-dose(6mg) Sinofuan group.The mice's general states,body weight and survival time were observed.The cumulative ascites volume and number of tumor cells were calculated.The apoptosis rates and cell cycle of tumor cells were analyzed with flow cytometry.Results Compared with the control group,the three Sinofuan groups showed better general state,slower body weight increase and longer medium survival(16.0,20.0,20.0 verse 13.0 days;P0.01).Ascites accumulation of three Sinofuan groups(low-,medium-,high-dose) were 9.2±0.9ml,5.6±1.0ml and 5.9±1.1ml;the count of ascites tumor cells per milliliter were(10.11±1.31)×108,(7.25±1.00)×108 and(7.47±1.11)×108,all of which were lower than that of control group(13.3±1.5ml and 14.73±1.25×108),with significant differences(P0.01).The apoptosis rates were 14.8±1.4%,23.9±2.6% and 21.5±3.1% respectively,and S phase percentages were 31.23±3.26%,50.17±4.60% and 47.08±4.11%,all of which were higher than that of control group(7.6±1.9% and 20.57±4.08%),showing significant differences(P0.01).The differences between the medium-and high-dose group were not significant(P0.05).Conclusion Sinofuan,especially in medium-dose,shows a good effect and safety in the treatment of the mice bearing H22 ascites tumors.It suggests that Sinofuan may be applied to treat the malignant ascites and peritoneal metastases in appropriate doses.