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2010•Chinese Journal of Public Health EngineeringRequires access

Determination of Microcystins in Water by Ultra High Performance Liquid Chromatography-Mass Spectrometry

Huang Wen-pen

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Abstract

Objective To establish a method for microcystins in water by ultra-high performance liquid chromatography-tandem mass spectrometry.Methods Ultra high performance liquid chromatography-mass spectrometry combining with solid phase extraction has been employed to enhance the accuracy in the determination of microcystins,such as microcystin-LR.Results The detection limits was 0.04 μg/L,the linear range was 10-1 000 μg/L,average recovery rate was 97.20%,average RSD was 4.71%.Conclusion The results show that the rapid,accurate and sensitive method is suitable for analyzing and monitoring microcystins in water.

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What this paper is about

Objective To establish a method for microcystins in water by ultra-high performance liquid chromatography-tandem mass spectrometry.Methods Ultra high performance liquid chromatography-mass spectrometry combining with solid phase extraction has been employed to enhance the accuracy in the determination of microcystins,such as microcystin-LR.Results The detection limits was 0.04 μg/L,the linear range was 10-1 000 μg/L,average recovery rate was 97.20%,average RSD was 4.71%.Conclusion The results show that the rapid,accurate and sensitive method is suitable for analyzing and monitoring microcystins in water.

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Available abstract

Objective To establish a method for microcystins in water by ultra-high performance liquid chromatography-tandem mass spectrometry.Methods Ultra high performance liquid chromatography-mass spectrometry combining with solid phase extraction has been employed to enhance the accuracy in the determination of microcystins,such as microcystin-LR.Results The detection limits was 0.04 μg/L,the linear range was 10-1 000 μg/L,average recovery rate was 97.20%,average RSD was 4.71%.Conclusion The results show that the rapid,accurate and sensitive method is suitable for analyzing and monitoring microcystins in water.

Key concepts: Chromatography, Mass spectrometry, Chemistry, Extraction (chemistry), High-performance liquid chromatography, Solid phase extraction, Detection limit, Tandem mass spectrometry

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