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2012•Zhongguo shiyan fangjixue zazhiRequires access

Studies on HPLC Fingerprint of Shuangshan Granules

Yuan Zhou

Open publisher page 1 citations

Abstract

Objective:HPLC chromatographic fingerprints for Shuangshan granules were developed in order to evaluate the internal quality.Method: HPLC analysis was performed at 25 ℃ by using a Diamonsil C18 column(4.6 mm×250 mm,5 μm),with the gradient elute of mobile phase consisting of acetonitrile-0.1% glacial acetic acid at the flow rate of 1.0 mL · min-1.The UV detection was set at 340 nm.Result: Chlorogenic acid was used as the reference substance in the HPLC chromatographic fingerprint of Shuangshan Granules,which showed 13 common peaks and the similarity was 0.98 among 10 samples.Conclusion: This method was accurate,repeatable and useful for the quality control of Shuangshan granules.

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What this paper is about

Objective:HPLC chromatographic fingerprints for Shuangshan granules were developed in order to evaluate the internal quality.Method: HPLC analysis was performed at 25 ℃ by using a Diamonsil C18 column(4.6 mm×250 mm,5 μm),with the gradient elute of mobile phase consisting of acetonitrile-0.1% glacial acetic acid at the flow rate of 1.0 mL · min-1.The UV detection was set at 340 nm.Result: Chlorogenic acid was used as the reference substance in the HPLC chromatographic fingerprint of Shuangshan Granules,which showed 13 common peaks and the similarity was 0.98 among 10 samples.Conclusion: This method was accurate,repeatable and useful for the quality control of Shuangshan granules.

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Available abstract

Objective:HPLC chromatographic fingerprints for Shuangshan granules were developed in order to evaluate the internal quality.Method: HPLC analysis was performed at 25 ℃ by using a Diamonsil C18 column(4.6 mm×250 mm,5 μm),with the gradient elute of mobile phase consisting of acetonitrile-0.1% glacial acetic acid at the flow rate of 1.0 mL · min-1.The UV detection was set at 340 nm.Result: Chlorogenic acid was used as the reference substance in the HPLC chromatographic fingerprint of Shuangshan Granules,which showed 13 common peaks and the similarity was 0.98 among 10 samples.Conclusion: This method was accurate,repeatable and useful for the quality control of Shuangshan granules.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Chlorogenic acid, Acetic acid, Fingerprint (computing), Gradient elution, Granule (geology)

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