2007•ZhongchengyaoRequires access

HPLC fingerprint analysis of Jiketing Granules

Zhao Lu

Abstract

AIM: To establish the fingerprint analysis method of Jiketing Granules(Fructus Forsythiae,Radix Scutellariae,Radix Bupleuri,etc) by HPLC-UV.METHODS: Aanalysis was performed on an alltima C_(18)(4.6 mm×250 mm,5 μm) column with a acetonitrile-0.1% acetic acid gradient.Detection time was 55 min.The flow rate was 1.0 mL/min.The montoring wavelength was changed.The colunm temperature was at 30℃. RESULTS: 21 peaks were separated on HPLC fingerprint in Jiketing Granules. CONCLUSION: The method is reliable,accurate and can be used as a quality control for Jiketing Granules.

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Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Fingerprint (computing), Radix (gastropod), Gradient elution, Acetic acid, Biochemistry