Fas&FasL、C-myc expression and testicular germ cell apoptosis in experimental cryptorchidism

Purpose:To investigate FasFasL、C myc expression and testicular germ cell apoptosis in experimental cryptorchidism.Method:Immature rats (22 days old Sprague Dawley) were subjected to unilateral cryptorchidism. Using terminal deoxynucleotidyl transferase mediated d UTP nick end Labeling (TUNEL) for the detection of germ cell apoptosis; Using immunohistochemical SP method for the detection of FasFasL、C myc expression. Result:①At the third day after operation, as compared to the contraposition control, neither the number of apoptotic germ cell, nor the augment of FasFasL、C myc expression was highly notable (P 0.05 ) in the cryptorchidism. The number of apoptotic germ cell increased markedly (P 0.01 ) and the augment of FasFasL、C myc expression was highly notable (P 0.01 ) in the cryptorchidism as compared to the contraposition control at the seventh day after operation. ②There is a considerable correlation coefficient between the expression of C myc and the respective expression of FasFasL at the third day and the sevConclusion:Enhanced expression of FasFasL、C myc might be involved in germ cell apoptosis in cryptorchidism; testicular germ cell apoptosis in experimental cryptorchidism occurs by Fas FasL mediated apoptosis in association with C myc.