2002•Biotechnology(Faisalabad)Requires access

Screening of high avermectin producing strains from S.avermitilis Y20

Lili Liang

Abstract

Objective:Study the characteristics of S.avermitilis Y20 and obtain high avermectin producing strains.Method:The types of colony and fermentation properties were studied with using S avermitilis Y20 as original strain.The mutagenesis methods are using UV,HNO 3 and NTG to treat S.avermitilis Y20 and using L-Ile as inducer.Result:The high avermectin producing strain H336 with avermectin titer up to 852 μg/mL was obtained.Conclusion:The mutant fermentation products are stable and the avermectin product is more than 10 times compared with original strain.

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Key concepts: Streptomyces avermitilis, Avermectin, Strain (injury), Chemistry, Inducer, Mutant, Biochemistry, Biology