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Directional screening of high producing strain of avermectin B1a component

Qiao Jian-jun

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Abstract

Streptomyces avermitilis strain 1-17 producing avermectin was mutated with UV and NTG on spores,then induced and selected by L-Ile,an avermectin B1a high-yielding strain 3-6 was selected,which produced 12.86% more avermectin Bla than the parent strain.Subculture test indicated that the heredity characteristics of strain 3-6 was stable.The results showed that it was an effective way to obtain high-producing strain of avermectin B1a component by using UV,NTG as mutagen and L-Ile as inducer.

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What this paper is about

Streptomyces avermitilis strain 1-17 producing avermectin was mutated with UV and NTG on spores,then induced and selected by L-Ile,an avermectin B1a high-yielding strain 3-6 was selected,which produced 12.86% more avermectin Bla than the parent strain.Subculture test indicated that the heredity characteristics of strain 3-6 was stable.The results showed that it was an effective way to obtain high-producing strain of avermectin B1a component by using UV,NTG as mutagen and L-Ile as inducer.

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Available abstract

Streptomyces avermitilis strain 1-17 producing avermectin was mutated with UV and NTG on spores,then induced and selected by L-Ile,an avermectin B1a high-yielding strain 3-6 was selected,which produced 12.86% more avermectin Bla than the parent strain.Subculture test indicated that the heredity characteristics of strain 3-6 was stable.The results showed that it was an effective way to obtain high-producing strain of avermectin B1a component by using UV,NTG as mutagen and L-Ile as inducer.

Key concepts: Avermectin, Streptomyces avermitilis, Strain (injury), Mutagen, Chemistry, Biology, Streptomyces, Biochemistry

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