Screening and Identification of Antagonistic Bacteria against Rhizoctonia solani in Cotton Rhizosphere

48 antagonistic bacteria screened from cotton rhizosphere were antibiotic against Rhizoctonia solabni Kuhn.Four isolates,MK48,MK49,MK50 and MK51 showed strong and stable antibiosis.Based on the results of morphologic characteristics,physiological and biochemical properties and sequence analysis of 16S rDNA,MK48 was identified as Brevibacillus brevis,MK49 and MK51 were identified as Bacillus subtilis,MK50 was identified as Pseudomonas aeruginosa.The 16S rDNA sequences of MK48,MK49,MK50 and MK51 have been submitted to GenBank and the registration number are EU272855,EU272856,EU272857 and EU272865.