Screening and identification of antagonistic actinomycete against Rhizoctonia solani

Four hundred and six actinomycetes were isolated from sixty eight soil samples which were collected from different areas of Jinhua.Forty-two strains had been proved to have antagonistic activity against Rhizoctonia solani by confronting cultivation on PDA plates.Among them,the strain Sh-43 exhibited the strongest antibiosis capacity with an inhibition zone of 28.3 mm.The strain Sh-43 was identified as Streptomyces hygroscopicus on the cultural,morphological,physiological,biochemical characteristics and 16S rDNA gene sequence analysis.