Induction of apoptosis by all trans-retinoic acid in bladder tumor T24 cell line

Purpose:To explore the all trans retinoic acid(ATRA) on induction of apoptosis in human bladder transitional cell carcinoma cell line T24.Methods: The apoptosis of T24 cell line was studied by fluorescence microscopy, flow cytometry and DNA agarose gel electrophoresis.Results:After T24 cells were treated by 3×10 -6 mol/L ATRA six days, the apoptotic rates of T24 cells were 15.20 % in treated group and 0.01 % in control group respectively under fluorescence microscopy(P 0.05 ); the apoptotic rates of T24 cells were 15.31 % in treated group and 1.49 % in control group separately on flow cytometry ; a characteristic DNA “ladder” on agarose gels was observed in treated group.Conclusions:The apoptosis of human bladder transitional cell carcinoma cell line T24 can be induced by ATRA.