Study the Effects on the Growth of Multiple Carcinoma Cells by All Trans Retinoic Acid

objective To treat prostatic carcinoma cell line DU-145?breast cancer cell line MCF-7 and promyeelocytic cell line HL-60 by all trans retinoic acid(ATRA), to observe the change of tumor cell's growth. Methods DU-145 cells?HL-60 cells and MCF-7 cells were treated with ATRA(10-5mol/L)for 36h and 72h, observed changes of the cell's shape and ultrastructure by optical and electron microscope. Analyzed the effect of retinoic acid on the cycle of tumor cells by flow cytometry FCM. Results After treated with ATRA, The results showed that all kinds of carcinoma cells DU-145 grow slowly, some of the carcinoma cell's ultrastructure appeared early apoptotic changes, including cell shrinkage, nuclear condensation. After 72h treated with ATRA, some apoptotic cells were detected,and a typical subdiploid peak were observed by flow cytometry. Conclusions Retinoic acid can induce multiple carcinoma cells to apoptosis. The apoptotic process has a time -depending character, and also depand on the concentration of retinoic acid.