Comparison of the Methods and Results of Determining the Activities of Proteolytic Enzymes Used in Leather Making

The methods of determining the activities of proteolytic enzymes used in Leather making, such us Folin method, Lhlein-Volard method (LVU) and the method of transforming coefficient of trypsinase to casein, were introduced. The above three methods were applied to determine 21 proteolytic enzymes analysis,which are used in leather making processes, including high content trypsinases, microorganic proteolytic enzymes and some industrial leather enzymes such as soaking enzymes, liming enzymes , bating enzymes as well. The results show that the activity values of the Folin method and the LVU method reacting under the same pH and temperature (pH8.2, temperature 37℃) are almost the same.