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2007•Chinese Journal of Public HealthRequires access

Preparation and primary purification of rabbit polyclonal antibodies against human Cap43 protein

Yin Yuan-qin

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Abstract

Objective To obtain an anti-Cap43 polyclonal antibody.Methods Peptide with 30 amino acids as hapten was coupled with mcKLH using carbodiimide(EDC) coupling chemical technique to form a coupling agent(peptide-mcKLH),then rabbits were immunized;To prepare anti-Cap43 polyclonal antibody.Valence of antibody was detected by dot-ELISA.The polyclonal antiserum was purified by caprylic acid-ammonium sulfate precipitation,then,the purity of antigen was detected by SDS-PAGE.Results Rabbit polyclonal antiserum against human Cap43 protein was obtained after 11 weeks and the titer of the antiserum obtained in this experiment was up to 1:500.Conclusion Coupling hapten with giant molecular vehicle mcKLH by EDC can make it complete antigen that posessed immunogenicity and immunoreactivity.Immunizing rabbits with the hapten-mcKLH was used to obtain the antibody against Cap43.

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Objective To obtain an anti-Cap43 polyclonal antibody.Methods Peptide with 30 amino acids as hapten was coupled with mcKLH using carbodiimide(EDC) coupling chemical technique to form a coupling agent(peptide-mcKLH),then rabbits were immunized;To prepare anti-Cap43 polyclonal antibody.Valence of antibody was detected by dot-ELISA.The polyclonal antiserum was purified by caprylic acid-ammonium sulfate precipitation,then,the purity of antigen was detected by SDS-PAGE.Results Rabbit polyclonal antiserum against human Cap43 protein was obtained after 11 weeks and the titer of the antiserum obtained in this experiment was up to 1:500.Conclusion Coupling hapten with giant molecular vehicle mcKLH by EDC can make it complete antigen that posessed immunogenicity and immunoreactivity.Immunizing rabbits with the hapten-mcKLH was used to obtain the antibody against Cap43.

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Available abstract

Objective To obtain an anti-Cap43 polyclonal antibody.Methods Peptide with 30 amino acids as hapten was coupled with mcKLH using carbodiimide(EDC) coupling chemical technique to form a coupling agent(peptide-mcKLH),then rabbits were immunized;To prepare anti-Cap43 polyclonal antibody.Valence of antibody was detected by dot-ELISA.The polyclonal antiserum was purified by caprylic acid-ammonium sulfate precipitation,then,the purity of antigen was detected by SDS-PAGE.Results Rabbit polyclonal antiserum against human Cap43 protein was obtained after 11 weeks and the titer of the antiserum obtained in this experiment was up to 1:500.Conclusion Coupling hapten with giant molecular vehicle mcKLH by EDC can make it complete antigen that posessed immunogenicity and immunoreactivity.Immunizing rabbits with the hapten-mcKLH was used to obtain the antibody against Cap43.

Key concepts: Polyclonal antibodies, Antiserum, Hapten, Antibody, Immunogenicity, Antigen, Chemistry, Peptide

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